American Society of Hematology

Fig. 8.

$Fig. 8. Hematopoietic potential of VE-cadherin+4-integrin+ cells sorted from yolk sac and embryonic body proper of 9.5 dpc mouse embryo. The VE-cadherin+4-integrin−, VE-cadherin+4-integrin+, and VE-cadherin−4-integrin+ cells were sorted from yolk sac and embryo proper of 9.5 dpc mouse embryos as shown in Fig 7. CD45+and TER119+ cells were excluded from the sorting gates. Sorted cells were cultured for 7 days in a matrix gel containing type I collagen in the presence of IL-3, Epo, G-CSF, and MGF. Morphology of hematopoietic cell colonies were examined by May-Gruenwald Giemsa staining (A). Colonies consisted of granulocytes (g) and erythrocytes (e) are observed. The bar represents 100 μm. (B) Frequency of hematopoietic colony-forming cells in the indicated cell fractions derived from yolk sac and embryonic body proper. Error bars indicate standard deviations for three independent determinations.$

Hematopoietic potential of VE-cadherin⁺4-integrin⁺ cells sorted from yolk sac and embryonic body proper of 9.5 dpc mouse embryo. The VE-cadherin⁺4-integrin⁻, VE-cadherin⁺4-integrin⁺, and VE-cadherin⁻4-integrin⁺ cells were sorted from yolk sac and embryo proper of 9.5 dpc mouse embryos as shown in Fig 7. CD45⁺and TER119⁺ cells were excluded from the sorting gates. Sorted cells were cultured for 7 days in a matrix gel containing type I collagen in the presence of IL-3, Epo, G-CSF, and MGF. Morphology of hematopoietic cell colonies were examined by May-Gruenwald Giemsa staining (A). Colonies consisted of granulocytes (g) and erythrocytes (e) are observed. The bar represents 100 μm. (B) Frequency of hematopoietic colony-forming cells in the indicated cell fractions derived from yolk sac and embryonic body proper. Error bars indicate standard deviations for three independent determinations.

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