Fig. 6.
Fig. 6. Southern blot analysis of MM tumors using 11q13 probes. (A) DNA was digested with HindIII restriction enzyme and hybridized with the 193 bp probe (see Materials and Methods) located in proximity of the KMS-12 breakpoint16; a rearrangement was observed in one tumor (case LB104; see text). (B) DNA was digested withBamHI restriction enzyme and hybridized with the A/H 0.6 kb probe located in proximity of the LB411 breakpoint. (C) DNA was digested with HindIII restriction enzyme and hybridized with the B/R 0.4 kb probe located in proximity of U266 breakpoint. The rearrangement patterns of KMS12, LB411, and U266 with the specified 11q13 probes are respectively shown in (A, B, and C). Germ-line fragments are indicated by dashes. The diagrams represent the 11q13 germ-line regions; probes used in the Southern blot analyses are shown. Restriction enzyme symbols: H, HindIII; B, BamHI; R,EcoRI; A, AvaI.

Southern blot analysis of MM tumors using 11q13 probes. (A) DNA was digested with HindIII restriction enzyme and hybridized with the 193 bp probe (see Materials and Methods) located in proximity of the KMS-12 breakpoint16; a rearrangement was observed in one tumor (case LB104; see text). (B) DNA was digested withBamHI restriction enzyme and hybridized with the A/H 0.6 kb probe located in proximity of the LB411 breakpoint. (C) DNA was digested with HindIII restriction enzyme and hybridized with the B/R 0.4 kb probe located in proximity of U266 breakpoint. The rearrangement patterns of KMS12, LB411, and U266 with the specified 11q13 probes are respectively shown in (A, B, and C). Germ-line fragments are indicated by dashes. The diagrams represent the 11q13 germ-line regions; probes used in the Southern blot analyses are shown. Restriction enzyme symbols: H, HindIII; B, BamHI; R,EcoRI; A, AvaI.

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