FISH analysis of the 11q13 breakpoints in MM tumors with a cytogenetically detectable t(11;14)(q13;q32) chromosome translocation. (A) FISH analysis of the KMS12 cell line: (1) partial metaphase cohybridized with chromosome 11 (green) and chromosome 14 (red) painting probes; (2) partial metaphase cohybridized with cosmid I4 (MTC-green) and C1 (red) probes; (3) partial metaphase cohybridized with YAC 961-H-2 (red) and C1 (green) probes. DAPI counterstaining is shown for each chromosome. (B) Double-color interphase FISH analysis of MM tumor AC97. Left: colocalized signals in cohybridization experiments with YAC 744-F-12 (green) and pHS11 (red) probes; right: dissociated signals in cohybridization experiments with pHS11 (red) and cyclin D1 (green) probes. See scheme in Fig 1for the probes used.