Fig. 8.
Fig. 8. Reactivation of HHV-6B in SAS527. HHV-6B–inoculated SAS527 cells were cultured in the presence of TPA at a concentration of 1.6 × 10−7 mol/L for 7 days. The cells were then observed with an inverted microscope for detection of CPE (A) and analyzed by indirect immunofluorescence with HHV-6–seropositive human serum (B). The cells were also sonicated and added to PHA-stimulated cord blood lymphocytes. After 6 days, the cells were observed with an inverted microscope for detection of CPE (C) and analyzed by indirect immunofluorescence with HHV-6–seropositive human serum (D).

Reactivation of HHV-6B in SAS527. HHV-6B–inoculated SAS527 cells were cultured in the presence of TPA at a concentration of 1.6 × 10−7 mol/L for 7 days. The cells were then observed with an inverted microscope for detection of CPE (A) and analyzed by indirect immunofluorescence with HHV-6–seropositive human serum (B). The cells were also sonicated and added to PHA-stimulated cord blood lymphocytes. After 6 days, the cells were observed with an inverted microscope for detection of CPE (C) and analyzed by indirect immunofluorescence with HHV-6–seropositive human serum (D).

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