Effects of different concentrations of platelets and neutrophils on the formation of 5-oxo-eicosanoids. Mixtures of platelets and neutrophils were incubated for 30 minutes at 37°C with A23187 (5 μmol/L) and PMA (30 nmol/L). The products were quantitated by RP-HPLC with PGB2 as an internal standard as shown in Fig 1. (A) Formation of 5-oxo-12-HETE (•), 8-trans-5-oxo-12-HETE (▴), and 5-oxo-ETE (□) by platelets (2 × 108/mL) in the presence or absence of various concentrations of neutrophils. (B) Formation of 5-oxo-12-HETE (•), 8-trans-5-oxo-12-HETE (▴), and 5-oxo-ETE (□) by neutrophils (5 × 106/mL) in the presence or absence of various concentrations of platelets. The inset shows the formation of 5-HETE (•) and LTB4 (○) by neutrophils (5 × 106/mL) in the presence or absence of various concentrations of platelets. The results are means ± standard error (SE) of experiments on mixtures of platelets and neutrophils from four different subjects.
