Western blotting analysis of the fractions obtained by sucrose gradient ultracentrifugation of Raji cell detergent lysate. The blots were immunostained by Abs to CD10, Lyn, and CD45 (a control protein that is not a component of the large buoyant complexes). (a) The buoyant fraction from the top of the gradient; (b) the bottom fraction. The buoyant fraction contained also large amounts of GPI-anchored proteins (CD48, CD55, and CD59; not shown).