Fig. 9.
Fig. 9. TCR rearrangements in the tumor cell lines. Southern blot analysis of tumor cell lines from groups A, B, C, and D was performed. Genomic DNA from each of the cell lines was cut to completion withHindIII, and analyzed by Southern blotting using a probe specific for TCR Cβ1 and Cβ2. Note that the cell lines in group A have a germline configuration of Cβ2, while those in groups B, C, and D have an altered or absent Cβ2 band, indicating that clonal rearrangement of the TCR has occurred. RW-4 (lane 7) represents DNA from an embryonic stem cell line with germline configurations of Cβ1 and Cβ2.

TCR rearrangements in the tumor cell lines. Southern blot analysis of tumor cell lines from groups A, B, C, and D was performed. Genomic DNA from each of the cell lines was cut to completion withHindIII, and analyzed by Southern blotting using a probe specific for TCR Cβ1 and Cβ2. Note that the cell lines in group A have a germline configuration of Cβ2, while those in groups B, C, and D have an altered or absent Cβ2 band, indicating that clonal rearrangement of the TCR has occurred. RW-4 (lane 7) represents DNA from an embryonic stem cell line with germline configurations of Cβ1 and Cβ2.

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