American Society of Hematology

Fig. 1.

$Fig. 1. Intracellular IFN-γ staining in cord blood NK cells, CD4+, and CD8+ T cells. NK cells with CD3−/CD16+/CD56+ phenotype isolated by fluorescence-activated cell sorting using FACS vantage flow cytometer (purity >90%), together with CD4+ (purity >98%) and CD8+ (purity >85%) separated from the CD16/56-negative fraction by positive immunomagnetic separation were separately stimulated and stained for cytokine production capability as reported.2 Representative histograms are shown with respective percentages of IFN-γ+cells. Mean populations (±SD) of IFN-γ–producing NK cells (n = 9), CD4+ (n = 17), and CD8+ (n = 8) T cells in cord blood are 19.12% ± 9.53%, 1.15% ± 0.62%, and 3.70% ± 1.58%, respectively.$

Intracellular IFN-γ staining in cord blood NK cells, CD4⁺, and CD8⁺ T cells. NK cells with CD3⁻/CD16⁺/CD56⁺ phenotype isolated by fluorescence-activated cell sorting using FACS vantage flow cytometer (purity >90%), together with CD4⁺ (purity >98%) and CD8⁺ (purity >85%) separated from the CD16/56-negative fraction by positive immunomagnetic separation were separately stimulated and stained for cytokine production capability as reported.2 Representative histograms are shown with respective percentages of IFN-γ⁺cells. Mean populations (±SD) of IFN-γ–producing NK cells (n = 9), CD4⁺ (n = 17), and CD8⁺ (n = 8) T cells in cord blood are 19.12% ± 9.53%, 1.15% ± 0.62%, and 3.70% ± 1.58%, respectively.

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