(A) Expression of c-Myc in CTCL cell lines studied by Western blotting with a polyclonal c-Myc antiserum (Santa Cruz Biotechnology). C, positive control with a 65-kD recombinant c-Myc–Glutathione-S-transferase (GST) fusion protein (Santa Cruz Biotechnology). Lanes 1 to 3, nuclear extracts from HUT78 (lane 1), MyLa (lane 2), and SeAx (lane 3) cells. The expected position of the differently modified c-Myc proteins (approximately 60 to 65 kD) is indicated on the right by an asterisk. The different bands in the area may result from differently phosphorylated forms of c-Myc. (B) Expression of Max in CTCL cell lines tested by a polyclonal Max antibody (Santa Cruz Biotechnology). M, marker; lanes 1 to 3, nuclear extracts from HUT78 (lane 1), MyLa (lane 2), and SeAx (lane 3) cells. The positions of the two forms of the Max protein are indicated on the right.