DGGE analysis of IgH CDR3 PCR products from microdissected nodal and splenic marginal zones. Lane 1, lanes 2 and 3, lanes 4 to 6, lanes 7 to 8, lanes 9 to 10, and lane 11 represent cases 5, 6, 2, 4, 7, and 1, respectively. Two patterns are observed on the denaturing gradient gel: a smear indicating polyclonal IgH gene rearrangements in lanes 1, 5, 6, and 9; and one or multiple bands indicating clonal IgH gene rearrangements in lanes 2 to 4, 7, 8, 10, and 11. Lanes 7 and 8 display comigrating bands indicating the presence of the same B-cell clone in the respective marginal zones. Sequence analysis of these IgH CDR3 products confirmed the presence of the same clone.