Growth and differentiation of cells expressing mLALA and mA mutants. (A) Cell-proliferation of 32D.cl8.6 clones expressing WT (▴; thick line), mDA (▪; thick dashed line), mLALA (▵; thin line), and mA (□; thin dashed line) receptors. Data represent growth of individual mLALA and mA clones, whereas for WT and mDA the mean growth of three clones is shown as a reference. (B) Maturation of 32D.cl8.6 cells expressing wild-type or mutant G-CSF receptors, as indicated in (A), expressed as the percentage of living cells showing signs of maturation at each time point. Data represent the mean of three clones. (C) Morphological features of 32D[WT] cells in the presence of IL-3 or representative clones expressing wild-type or mutant G-CSF-R after 7 days of exposure on G-CSF.