Cell surface expressions of chemokine receptors in monocyte-derived DCs. (A) Chemokine receptors expression levels in the transfectants expressing CCR-1 or CCR-3. The transfectants were stained with anti–CCR-1 MoAbs, anti–CCR-3 MoAbs, biotin-conjugated anti–CXCR-4 MoAbs, or FITC-conjugated anti–CCR-5 MoAb (thick lines) or FITC-conjugated mouse Ig (thin lines) for 30 minutes at 4°C. In an indirect staining, the cells were subsequently stained with FITC-conjugated antimouse IgG or FITC-conjugated avidin for 30 minutes at 4°C. (B) Expression levels of chemokine receptors in monocyte-derived DCs. Monocyte-derived DCs were stained with MoAbs to the respective chemokine receptors as described above. The cell surface expression was analyzed by flow cytometry. The results are representative of three experiments performed with similar results.