Detection of mRNA for EBNA-1 and LMP-1. The expression of EBNA-1, LMP-1, and G3PDH mRNA was examined by RT-PCR. Lane 1, Raji cells; lane 2, HB4C5 cells; lane 3, HTD8 cells; and lane 4, Molt-4 cells. The PCR-amplified products were run on agarose gels and assayed for hybridization to specific internal probes (EBNA-1 probe: 5′-TGACGGAGATGAAGGAGGTG-3′; LMP-1 probe: 5′-TTGTGCTGTTCATCTTTGGC-3′) by Southern blotting.