ANNEXIN VIII expression in HL-60 cells engineered to express E2A-HLF. (A) Immunoblot analysis of HL-60 cells transfected with zinc-regulated pMT-CB6+/E2A-HLF (HL-60[E2A-HLF]24 cells). Cell lysates were prepared from cells grown in medium lacking exogenous zinc (lane 1) and at serial intervals after the addition of zinc (100 μmol/L) to the medium (lanes 2 through 8). Control HL-60 cells transfected with the empty pMT vector (HL-60 [pMT]) did not express E2A-HLF, whether cells were grown in the absence (lane 9) or presence (lane 10) of zinc. Northern blot analysis of poly(A) RNA (1 μg per lane) prepared from HL-60(E2A-HLF)24 or HL-60(pMT) cells grown under the same conditions hybridized with either (B) ANNEXIN VIII or (C) SRPUL cDNA probes. (D) The blot was stripped and rehybridized with a control β-ACTIN probe.