Upregulation of ANNEXIN VIII and SRPULcDNAs by E2A-HLF. Northern blot analysis of poly(A) RNA (1 μg per lane) prepared from UOC-B1(dn)3 cells grown in zinc-free medium (lane 1) or in medium supplemented with zinc for the indicated times (lanes 2 through 8), followed by zinc depletion (lanes 9 through 12). Control experiments were performed with UOC-B1 cells transfected with an empty vector and grown in zinc-free medium (lane 13) or for 24 hours in the presence of zinc (lane 14). (A) The blot was hybridized with ANNEXIN VIII (F-5) (◂), (B)SRPUL (G-4) (◃), or (C) HLF cDNA fragments, and then stripped and rehybridized with a β-ACTIN probe (D) (•).