Fig. 1.
![Fig. 1. Immunoblot analysis of E2A-HLF and E2A-HLF(dn) proteins. Cell lysates were prepared from UOC-B1(dn)3 cells grown in the absence of zinc (lane 1) or in its presence (100 μmol/L) for the indicated intervals (lanes 2 through 7). After 24 hours, a portion of the cells were washed to remove zinc and cultured for the indicated intervals in the absence of the metal (lanes 8 through 11). Control UOC-B1 cells electroporated with the pMT empty vector (UOC-B1[pMT]) and grown for 24 hours in medium without (lane 12) or with 100 μmol/L zinc (lane 13).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/1/10.1182_blood.v93.1.321/5/blod40105001w.jpeg?Expires=1724271351&Signature=tvNxePY~Rhkc1dpyK9Tq4mJpg-av3l5Vs0J7PfR8fqZLBpCV2td~xf67mD17ao9A7s3LDjU1dE2XDhYh6lbHBK8W991yyYdC9P4OZcMDBR0nik1LS5cj6yns6GWDGYvz0jEPnuhR-c~m67LE~FQYg2PEylZXAjWCT~lrrsAwzhiVfIV6qIfsNv7s47KQJ-EcRBRBwIdNJC1w-X0tL7Vomv1yveceiXBkSUz1IkStdmBMadUX-AtpE92f42Kr9ZUzCcLmvhmVLY3bwUB9jD~4b39Fh8~fh6N~ZWoGzBw0sPrvssEH394fDqWQt1e7we7AvHZ114YfW5Wz~Mt46Ruf7w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Immunoblot analysis of E2A-HLF and E2A-HLF(dn) proteins. Cell lysates were prepared from UOC-B1(dn)3 cells grown in the absence of zinc (lane 1) or in its presence (100 μmol/L) for the indicated intervals (lanes 2 through 7). After 24 hours, a portion of the cells were washed to remove zinc and cultured for the indicated intervals in the absence of the metal (lanes 8 through 11). Control UOC-B1 cells electroporated with the pMT empty vector (UOC-B1[pMT]) and grown for 24 hours in medium without (lane 12) or with 100 μmol/L zinc (lane 13).
