RT-PCR analysis of D3 expression in cell lines and in normal hematopoietic cells. (A) Plasmids containing the full-length cDNAs for D3 and 204 were purified and amplified using D3/204 primers, which amplifies a 505-bp band visualized by ethidium bromide staining. The amplification products were then purified and treated with (+) or without (−) Cla I. RNA extracted from EML, EML cultured in IL-3/SCF/atRA for 6 days, MPRO, and EPRO cell lines were reverse transcribed with oligo (dT), amplified with D3/204 primers, and treated with (+) or without (−) Cla I. (B) RNA from normal hematopoietic cells was purified and analyzed for D3 expression by RT-PCR as indicated above.