Fig. 1.
(A) Northern blot analysis of RNA extracted from the EML and MPRO cell lines. The blots were probed with partial cDNA clones isolated by DDRT-PCR and reprobed with human β-actin. (B) Northern blot analysis of RNA extracted from MPRO, EPRO, and EML cell lines and EML cells treated with IFNγ for 16 hours or IL-3/SCF/atRA for 6 days. The blot was probed with the 3′ UTR of the D3 gene and reprobed with G3PDH. (C) Northern blot analysis of RNA extracted from EML cells treated for 1 or 2 days with (+) or without (−) CM, SCF, IL-3, and atRA. The blot was probed with the 3′ UTR of D3 gene and reprobed with G3PDH. (D) Northern blot analysis of RNA extracted from EML cells cultured for 0 to 144 hours in IL-3/BHK CM/atRA. The blot was probed with D3 (top), MPO (center), and G3PDH (bottom).