Fig. 3.
Fig. 3. Phenotype of adherent and nonadherent DC subsets from mesenteric and peripheral LN. Mesenteric and peripheral LN DC were purified as described, incubated for 90 minutes at 37°C, and the nonadherent and adherent fractions collected and analyzed for the expression of CD8 and LFA-1. The contour plots show the LFA-1 versus CD8 profiles of adherent and nonadherent DC after gating for CD11c+ cells. The dotted lines mark the lower limit defining the expression at high levels of the corresponding marker. These data are representative of four experiments with similar results.

Phenotype of adherent and nonadherent DC subsets from mesenteric and peripheral LN. Mesenteric and peripheral LN DC were purified as described, incubated for 90 minutes at 37°C, and the nonadherent and adherent fractions collected and analyzed for the expression of CD8 and LFA-1. The contour plots show the LFA-1 versus CD8 profiles of adherent and nonadherent DC after gating for CD11c+ cells. The dotted lines mark the lower limit defining the expression at high levels of the corresponding marker. These data are representative of four experiments with similar results.

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