Uptake of DiI-Ac-LDL and staining by anti–factor VIII antibody. (A and F) LE1SVO; (B and G) STR-2; (C and H) STR-4; (D and I) STR-10; (E and J) STR-12. (A through E) The cells were incubated with anti–factor VIII antibody and fluorescein-conjugated second antibody, and then examined under a confocal laser microscope (original magnification × 100). (F through J) The cells were incubated with DiI-acetylated LDL (10 mg/mL) at 37°C for 4 hours. After thorough washing, the cells were fixed and then evaluated for LDL uptake under a confocal laser microscope (original magnification × 100).