PCR analysis of the TCRBV expression using BV-specific primers showed that in both T-cell lines BV2⁺ and BV8S3⁺ T-cell clones were present. By limiting dilution, two different types of BV2⁺ T-cell clones 4F10 and 1F2, and one type of BV8S3⁺ T-cell clone were obtained. PCR mix A contained primers for TCR BV2, BV6, BV17, BV18, and BV21; mix B contained primers for TCR BV5S1, BV7, BV8, BV9, BV14, and BV23. Both BV2⁺ T-cell clones 4F10 and 1F2 show two bands because of the difference in running pattern of the single-stranded cDNA fragments after denaturation.