Fig. 2.
![Fig. 2. Internalization of surface-bound [125I] G-CSF by WT and mutant ▵716 G-CSFR complexes. Three days after transfection COS-7 cells were washed, incubated with 500 pmol/L [125I]-G-CSF for 2 hours at 4°C, then shifted to 37°C for the indicated times. Cells were returned to ice again for 2 hours, then washed to eliminate unbound ligand. Surface-bound ligand (▪) was determined after acid stripping in 0.5 mol/L NaCl/HCl (pH1.0) for 3 minutes. Internalized ligand (○) was measured after lysis of the cells in 1 mol/L NaOH. Data are expressed as a percentage of initial binding of G-CSF at 4°C. Values represent the mean of duplicate points. The data shown are from three independent experiments. Standard deviations are indicated by error bars.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/2/10.1182_blood.v93.2.440/4/blod40223002x.jpeg?Expires=1724243596&Signature=dXL56amnl9TLFVXX-1MmJ5OiAMRFexMV5NCzvcFWeX06E7tBzXtkeT~zReoaEpu1bBMZ7DuvSDTn8L2ZMn~FCq-c35XRsmmNJj5pF63XiQscJ65htvpAtlJGTi4NN-LLowRjj01ylI7PyBS1M~PZySEa~7jx5FkbGJ2KxSaEuLDOSLga5NL3kv2HFguBsNH4dVFYxPfP-MHn7mVOQTEF7uBubXetIJfj-Cahuc43EMo4KXRE2u2IqU9PYjAPyNwomJ0U3tInPM4nYofwo7X0ZI8TdsB42dtksxJ~VBmdq86W9tNMITuW69rJkbD6HCk03tPH0q1DZCAL2l52hZ7dfA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Internalization of surface-bound [125I] G-CSF by WT and mutant ▵716 G-CSFR complexes. Three days after transfection COS-7 cells were washed, incubated with 500 pmol/L [125I]-G-CSF for 2 hours at 4°C, then shifted to 37°C for the indicated times. Cells were returned to ice again for 2 hours, then washed to eliminate unbound ligand. Surface-bound ligand (▪) was determined after acid stripping in 0.5 mol/L NaCl/HCl (pH1.0) for 3 minutes. Internalized ligand (○) was measured after lysis of the cells in 1 mol/L NaOH. Data are expressed as a percentage of initial binding of G-CSF at 4°C. Values represent the mean of duplicate points. The data shown are from three independent experiments. Standard deviations are indicated by error bars.
