Elastase release from unstimulated neutrophils (Con) or neutrophils treated with chemotactic agents (10−7 mol/L PAF, 10−7 mol/L fMLP, 1% ZAP as a source of C5a, or 1 μg/mL IL-8) either in suspension (□) or while adherent to platelet monolayers (▪). Data are the mean ± SEM from three to eight experiments. ANOVA showed significant effect of treatment and of adhesion on release of elastase. Paired t-test showed a significant increase (#P < .05) in elastase released from neutrophils in suspension activated by C5a and IL-8 compared with control; a significant increase (**P < .01) in elastase released from surface-adherent neutrophils activated by fMLP, C5a, or IL-8 compared with control; a significant increase (+P < .05) in elastase released from rolling unstimulated control neutrophils compared with unstimulated control neutrophils in suspension.
