Fig. 3.
Phagocytosis of latex beads by G-CSF–elicited peripheral blood neutrophils from WT and βc-null mice. 105neutrophils were incubated in 1-mL cultures with 106 beads and either carrier or mGM-CSF, 4,000 U/mL, for 6 hours. Cytospin preparations were stained and 200 consecutive neutrophils were scored for number of cell-associated beads. The WPI was derived for each mouse by multiplying the number of neutrophils with 1, 2 to 3, 4, or ≥5 associated beads by 1, 2, 3, or 4, respectively, and dividing the total score by the number of neutrophils examined. Results for two mice of each genotype are shown and similar results were obtained in a further seven mice.
