Recognition of plasma and recombinant vWF by MoAbs. Plasma samples and conditioned media containing 0.01 U/mL vWF:Ag were incubated for 1 hour at 37°C into microtiter plate coated with (▪) MoAb 32B12 or (▧) MoAb 31H3. After washing, the bound vWF was detected with peroxidase-conjugated anti-vWF polyclonal antibodies. Values are expressed as a percent of the absorbance obtained with normal plasma or WT rvWF. Each value is the mean (± SD) of three experiments.