Fig. 5.
Binding of differentially sulfated GAGs to PF4. GAGs were perfused over biotinylated PF4 immobilized on a streptavidin chip in BIAcore biosensor equipment. Binding was measured in resonance units (1 RU = 1 pg/mm2) using surface plasmon resonance. Binding of each GAG was measured in triplicate at each concentration for five different concentrations. Representative binding curves are shown for the following concentrations of GAGs: (A) desulfated heparin, 3.4 × 10−6 mol/L; (B) N-sulfated heparin, 3.4 × 10−6 mol/L; (C) O-sulfated heparin, 1.7 × 10−6 mol/L; (D) bovine kidney heparan sulfate, 8.33 × 10−7 mol/L; and (E) unmodified heparin, 8.33 × 10−7 mol/L.
