Phenotypic analysis of in vitro–generated DC. PBMNC were cultured in presence of CD40L, CD40L/IL-4, or GM-CSF/IL-4. Dot plots (A) show the forward- and side-scatter profiles of the cells. Histograms (B) show expression of indicated cell surface molecules after 7 days of culture. Solid histograms: labeling with isotype matched irrelevant MoAb.