Phenotypic analysis of in vitro–generated DC. PBMNC were cultured in presence of Flt3L/IL-4 (bold solid line), Flt3L/IL-4/ TNF- (thin solid line), or Flt3L/IL-4/CD40L (dotted line). Overlay diagrams show expression of indicated molecules after 7 days of culture. Solid histograms: labeling with isotype matched irrelevant MoAb.