Specific amplification of normal and [T¹⁸⁴⁶]-GPIIIa alleles. A DNA fragment of 148 bp encompassing exon 11 and intronic flanking regions of GPIIIa was amplified from genomic DNA of a control, the proband, her parents, and her brother. Each DNA was amplified using a sense primer whose 3′ end was complementary to either the normal sequence (Wt) or to the mutant base (Mut). The amplification products were electrophoresed in a 3% agarose gel and the DNA bands were stained with ethidium bromide.