FACS analysis of K562 clones transfected with cDNAs corresponding to Rh and/or band 3. (1) to (3) are data for K562/pBp and K562/B3 clone 1, Table 1, day 1. (4) to (6) are data for a K562/RhD+pBn clone (with expression levels close to the calculated average) and K562/RhD+B3 clone 1; all data are from Table 4. (7) to (9) are data for a K562/RhcE+pBn clone (with expression levels close to the calculated average) and K562/RhcE+B3 clone 1; all data are from Table 4. Antibodies used were: BRIC 6 (anti–band 3) in histograms 1, 4, and 7; BRIC 69 (anti-Rh30) in histograms 2, 5, and 8; LA18.18 (anti-RhGP) in histograms 3, 6, and 9. Rh30, Rh polypeptides; RhGP, Rh glycoprotein.