Phenotypic modifications induced by culture with RCC CM for 12 days. CD34⁺ cells cultured for 12 days with GM-CSF+TNF (▪) or in the presence of 10% RCC CM (□) were harvested and processed for staining with PE-conjugated MoAbs or uncoupled MoAb shown by PE-conjugated antirat Ig (CD115). Antimouse control antibody was shown in the first histogram. Antirat control antibody overlaps this control (data not shown). Results are representative of four experiments.