Fig. 2.
Fig. 2. Nucleotide sequence of the human c-kit promoter from −124/+37. The sequence is numbered from the 5′ transcription initiation site. The locations of the double-stranded oligonucleotides (XmaI-NaeI, XN, XN2, XN3) used in gel shift analysis (below) are indicated. The locations of potential Sp1 binding sites are indicated as lines above the sequence showing degree of homology to the consensus decanucleotide. The TT in the XN2mt oligonucleotide marks the location of an inserted 2-bp mutation (below). The +37 nucleotide is the most 3′ base of c-kit promoter sequence before the pGL2 polylinker in the reporter plasmids.

Nucleotide sequence of the human c-kit promoter from −124/+37. The sequence is numbered from the 5′ transcription initiation site. The locations of the double-stranded oligonucleotides (XmaI-NaeI, XN, XN2, XN3) used in gel shift analysis (below) are indicated. The locations of potential Sp1 binding sites are indicated as lines above the sequence showing degree of homology to the consensus decanucleotide. The TT in the XN2mt oligonucleotide marks the location of an inserted 2-bp mutation (below). The +37 nucleotide is the most 3′ base of c-kit promoter sequence before the pGL2 polylinker in the reporter plasmids.

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