Fig. 2.
![Fig. 2. Activation of v-Abl TK prevents reduction in Ψm after withdrawal of IL-3. (A [i]) Viable cells were defined in region R1 by exclusion of propidium iodide. (A [ii]) Example of the measurement of Ψm in IC.DP cells (0 hours) using DiOC(6)3 (solid histogram) and collapse of Ψm with 10 μmol/L mCCCP (open histogram). (B) Kinetics of changes in Ψm in IC.DP (squares) and IC2.9 (triangles) cells after withdrawal of IL-3. Cells were maintained at either 32°C (open symbols) or 39°C (solid symbols). Data were normalized against the mean DiOC(6)3 fluorescence intensity for each sample at 0 hours. Data points are the mean ± SEM of three duplicate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/12/10.1182_blood.v92.12.4545/5/blod42441002x.jpeg?Expires=1724235082&Signature=kGVhim5TOatx~ZWfSAD-I9cy~HtanxgVNhXxqJGgJ6qWZsQjXgAtzMgpPKVo99dGsruhjLmUAQDXtZBHzmmEswV6F~HlvbionsexHtmTCWfgInL92~c8wIPlxX2p4BYf4D4mWhUkvFmdckvDZM9pED1k4qON-iMYLklEbjwg6jOzMw7X7307kG0SJdmMinShNK87SvzGUmJ9uxcBB19x9uGdfL8tMcHi-CYUyi-u7rhcoXUDb~KBjqCVr0E0rCwwteqPnG9O45UdHNfGzhRMnkv31klsU53SST8DShzw7Ut5d3TVrWdk3lZyUwzgfh-tkle~lJyX-NtDUlMyYpOkwQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Activation of v-Abl TK prevents reduction in Ψm after withdrawal of IL-3. (A [i]) Viable cells were defined in region R1 by exclusion of propidium iodide. (A [ii]) Example of the measurement of Ψm in IC.DP cells (0 hours) using DiOC(6)3 (solid histogram) and collapse of Ψm with 10 μmol/L mCCCP (open histogram). (B) Kinetics of changes in Ψm in IC.DP (squares) and IC2.9 (triangles) cells after withdrawal of IL-3. Cells were maintained at either 32°C (open symbols) or 39°C (solid symbols). Data were normalized against the mean DiOC(6)3 fluorescence intensity for each sample at 0 hours. Data points are the mean ± SEM of three duplicate experiments.
