CD34⁺ cord blood cells in FTOC acquire a T-cell immunophenotype in the continuous presence of SCF, Flt-3L, IL-7, IL-1, and IL-15. CD34⁺ cells were sorted and cultured in fetal thymic lobes with the cytokines for 35 days. FTOC were then harvested, and T-cell differentiation was assessed by flow cytometric analysis using the indicated antibodies.