Fig. 2.
Fig. 2. Nucleotide sequence of the 5′-flanking region of the endoglin gene. Numbers at the left margin refer to the transcription start site (+1) determined by primer extension analysis in this report. Arrowheads indicate consensus sites for specific restriction enzymes. (A) Sequence of the upstreamSacII/BamHI fragment (−2616 to −401). An Alu sequence located between −927 and −666 is underlined. (B) Sequence of the downstream BamHI/PvuII region (−400 to +672). Solid arrows indicate the transcription start sites reported here by primer extension: +1, +18, +47, and +48 (lung); +2 and +18 (placenta). Open arrows indicate the transcription start sites reported here by 5′ RACE: +9, and +78 (placenta); +39 and +78 (U937). The endoglin cDNA sequence previously described (Bellón et al16) is underlined with a dashed line. Consensus sequences for putative regulatory motifs include AP-2, Ets, GATA, NFκB, and Sp1. The location of the oligonucleotides PE#2 and PE#4 used for hybridization studies are indicated with a dotted overline. The derived aminoacid sequence of the signal peptide is shown underneath the coding region of the first exon using the three letter code. The nucleotide sequences of the 3.3-kb SacII/PvuII and 0.74-kbBamHI/BbrPI fragments have been assigned the EMBL/GeneBank accession nos. AF035753 and Y11653, respectively.

Nucleotide sequence of the 5′-flanking region of the endoglin gene. Numbers at the left margin refer to the transcription start site (+1) determined by primer extension analysis in this report. Arrowheads indicate consensus sites for specific restriction enzymes. (A) Sequence of the upstreamSacII/BamHI fragment (−2616 to −401). An Alu sequence located between −927 and −666 is underlined. (B) Sequence of the downstream BamHI/PvuII region (−400 to +672). Solid arrows indicate the transcription start sites reported here by primer extension: +1, +18, +47, and +48 (lung); +2 and +18 (placenta). Open arrows indicate the transcription start sites reported here by 5′ RACE: +9, and +78 (placenta); +39 and +78 (U937). The endoglin cDNA sequence previously described (Bellón et al16) is underlined with a dashed line. Consensus sequences for putative regulatory motifs include AP-2, Ets, GATA, NFκB, and Sp1. The location of the oligonucleotides PE#2 and PE#4 used for hybridization studies are indicated with a dotted overline. The derived aminoacid sequence of the signal peptide is shown underneath the coding region of the first exon using the three letter code. The nucleotide sequences of the 3.3-kb SacII/PvuII and 0.74-kbBamHI/BbrPI fragments have been assigned the EMBL/GeneBank accession nos. AF035753 and Y11653, respectively.

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