Fig. 1.
![Fig. 1. Specificity of the newly established MoAbs HSL11, HSL96, and HSL2 for human λ5, VpreB, and pre-BCR, respectively. A pre–B-cell line NALM6 (A), a pro–B-cell line RS4;11 (B), and X63.Ag8-653 cells transfected with either the λ5 or VpreB gene (C) were biosynthetically labeled with [35S]-methionine for 4 hours and lysed with 1% NP-40 lysis buffer. Detergent-soluble lysates were incubated with indicated MoAbs, and immunoprecipitates were analyzed by 13% SDS polyacrylamide gel electrophoresis under reducing conditions. The positions of μH, λ5, and VpreB are indicated by arrows, and a 16-kD band precipitated by HSL11 together with λ5 is indicated by an arrow head.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/92/11/10.1182_blood.v92.11.4317/5/blod42343001w.jpeg?Expires=1722721632&Signature=JjY~h~bqPgun8QC7bPit1q5gwCIoOwzItcOzPz3Yi1wqHWbWeeCA4Ya7tpdjI9eCPbUnBDDmpXRDWPwrsYegPKitG1379OpuTl7TWIk0BWZKn5VqHxNF9auSqMNzfwF2ZmHhm2q5ew1qEY0tqeMFXcWj2j6X-5sk8qXiudVsFLeZRsoxPIRsh17~KXj4pCf7w~OApmT9v~g~Td4aCDNvhpWjLwMvjHeVIah1w7sTam5nFHFcES8aEtJXNKYWMJZD-1Xg1EnpCAqdn7yvDaUb9sd9Frq7Nd6gZMUNERMU7KzgDLr33iBYntiWXx7XCpgOlAoc5mSqPxdkG5i9GwD0TA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Specificity of the newly established MoAbs HSL11, HSL96, and HSL2 for human λ5, VpreB, and pre-BCR, respectively. A pre–B-cell line NALM6 (A), a pro–B-cell line RS4;11 (B), and X63.Ag8-653 cells transfected with either the λ5 or VpreB gene (C) were biosynthetically labeled with [35S]-methionine for 4 hours and lysed with 1% NP-40 lysis buffer. Detergent-soluble lysates were incubated with indicated MoAbs, and immunoprecipitates were analyzed by 13% SDS polyacrylamide gel electrophoresis under reducing conditions. The positions of μH, λ5, and VpreB are indicated by arrows, and a 16-kD band precipitated by HSL11 together with λ5 is indicated by an arrow head.
