American Society of Hematology

Fig. 7.

$Fig. 7. Isoelectrofocusing separation of the Hb tetramers present in supernatant 1 and supernatant 2 (see Materials and Methods). Lane 1, markers for HbS A, F, S, and C(A2) (from the top down). Lane 2, supernatant 1 of a normal hemolysate (HbA, traces of HbF and low levels of HbA2; the bands in front of HbA are postranslational modifications of HbA). Lane 3, supernatant 1 of patient SSR (HbA, low amounts of HbF and HbA2 and the slowest fraction, HbS-Oman). Lane 4, supernatant 1 of patient ISH (high S-Oman, similar distribution to that in 3 but less loaded). Lane 5, supernatant 2 of a normal hemolysate (HbA and very little A2). Lane 6, supernatant 2 of patient SSR, dramatic reduction of HbA and large increase in HbS-Oman. Lane 7, supernatant of patient ISH, very similar results that in lane 6. For quantification, see the text.$

Isoelectrofocusing separation of the Hb tetramers present in supernatant 1 and supernatant 2 (see Materials and Methods). Lane 1, markers for HbS A, F, S, and C(A₂) (from the top down). Lane 2, supernatant 1 of a normal hemolysate (HbA, traces of HbF and low levels of HbA₂; the bands in front of HbA are postranslational modifications of HbA). Lane 3, supernatant 1 of patient SSR (HbA, low amounts of HbF and HbA₂ and the slowest fraction, HbS-Oman). Lane 4, supernatant 1 of patient ISH (high S-Oman, similar distribution to that in 3 but less loaded). Lane 5, supernatant 2 of a normal hemolysate (HbA and very little A₂). Lane 6, supernatant 2 of patient SSR, dramatic reduction of HbA and large increase in HbS-Oman. Lane 7, supernatant of patient ISH, very similar results that in lane 6. For quantification, see the text.

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