Inhibition of endogenous Evi-1 restores responsiveness of MOLM-1 cells to TGF-β. (A) MOLM-1 cells were treated with no (−), the sense (S), or the antisense (AS) oligonucleotide for Evi-1. Whole-cell extracts containing 100 μg of proteins were subjected to SDS-PAGE and immunoblotting with the anti–Evi-1 antibody. The amount of the Evi-1 protein expressed in these cells is shown. The arrow indicates the migration of the Evi-1 protein, and the positions of molecular-weight standards are shown at left. (B) Analysis of growth inhibition in response to TGF-β. MOLM-1 cells treated with the indicated oligonucleotide were subjected to a [³H]thymidine incorporation assay in the presence of different concentrations of TGF-β. Results are expressed as percentages relative to values observed in control cultures that did not receive TGF-β. Representative values from four independent experiments are shown.