Transfer of CD59 from RBC eluates (A) and HDL (B) to monkey RBC lacking cross reactive CD59 antigen. Monkey RBC was washed in PBS, incubated with RBC eluate or HDL for 1 hour at 37°C, then subsequently washed and stained with CD59-FITC. PI-PLC (1 U/mL) for 1 hour at 37°C treatment of samples of RBC eluate and HDL significantly decreased transfer.