Effect of accutin on spontaneous angiogenesis in vivo. CAMs of 10-day-old chick embryos were incubated with vehicle (A, control), GRGES (B, 1mmol/L), or indicated concentrations of accutin (C through F, 1, 2, 5, 10 μmol/L, all in 100 μL) for 48 hours, and then resected, fixed, and photographed with a stereomicroscope at 10× magnification.