T cells from GVHD mice generate normal proliferative and normal CTL response to HSV-1. GVHD mice display a Th2 cytokine response to HSV-1. (A) Nylon wool–separated T cells from GVHD mice respond well to HSV-1. 3H-Thymidine incorporation by recipient splenocytes in response to inactivated virus or media alone was measured. Groups consisted of two or three mice, and the mean and SEM are shown. This experiment is representative of three similar experiments. (B) HSV-1 CTL assays showed that GVHD and control mice generated similar CTL responses after HSV-1 infection. Lysis of mock-infected L929 targets was less than 20%. The data shown were from one of three similar experiments. (C) Splenocytes were cultured in vitro with ultraviolet irradiation-inactivated HSV-1 or with an extract from uninfected Vero cells, and IFN-γ production was measured from supernatants. The mean and standard error of three replicate wells are shown; data are representative of three similar experiments with three mice per group in each experiment. The quantity of IFN-γ produced by the extract control was below the limit of detection of the assay. Significant differences were detected between the extract and virus data (P ≤ .05, Student’s t-test). (D) Splenocytes were cultured in vitro with ultraviolet irradiation-inactivated HSV-1 or with an extract from uninfected Vero cells, and IL-4 production was measured from supernatants. Data from a single experiment is shown. Significant differences were detected between the extract and virus data (P ≤ .05, t-test). Results from two additional experiments measuring IL-5 were similar to the IL-4 experiment (data not shown).
