Decrease of porphyrin content in GATA-1.05 mutant hematopoietic cells. (A and B) Dianisidine staining of the wild-type and GATA-1.05 mutant cells in the day 8 floating fraction, respectively. (C) Color comparison of floating cell pellet at day 14. (D) Measurement of the porphyrin content in the cells. The fluorescence spectra in lysate of both wild-type and GATA-1.05 floating fraction showed the specific profile of porphyrins. The spectrum of the higher peak shows wild type and that with the lower peak shows the mutant results.