American Society of Hematology

Fig. 3.

$Fig. 3. Expression profiles of erythroid transcription factors in the OP9/ES cell differentiation system. Adherent and floating fractions were collected at time points indicated from the in vitro ES cell culture system. Concentrations of all the prepared cDNAs were adjusted to produce equal amounts of HPRT amplicon. Expression levels of mRNA encoding ɛy- and β-major globins were first measured by RT-PCR (A). The expression profiles of erythroid transcription factors during the differentiation of ES cells were then examined. The expression profiles are categorized into three groups. The first group includes GATA-1, p45, EKLF, and SCL (B); the second group consists of GATA-2 and c-myb (C); and the third group includes Nrf2 and GATA-3 (D). Each group is shown as a pair of boxes; the left box represents the adherent fraction and the right box represents the floating fraction. Highest expression for each factors is set to 100%.$

Expression profiles of erythroid transcription factors in the OP9/ES cell differentiation system. Adherent and floating fractions were collected at time points indicated from the in vitro ES cell culture system. Concentrations of all the prepared cDNAs were adjusted to produce equal amounts of HPRT amplicon. Expression levels of mRNA encoding ɛy- and β-major globins were first measured by RT-PCR (A). The expression profiles of erythroid transcription factors during the differentiation of ES cells were then examined. The expression profiles are categorized into three groups. The first group includes GATA-1, p45, EKLF, and SCL (B); the second group consists of GATA-2 and c-myb (C); and the third group includes Nrf2 and GATA-3 (D). Each group is shown as a pair of boxes; the left box represents the adherent fraction and the right box represents the floating fraction. Highest expression for each factors is set to 100%.

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