Chemotaxis assay using soluble TF and anti-TF MoAb. Lower wells of blind-well chambers were filled with control medium (No Attractant) or medium containing 10 nmol/L FMLP, 10 nmol/L FMLP + 10 μg/mL anti-TF MoAb VIC7, or 0.5 nmol/L soluble TF. Reverse-transmigrated MP collected from the apical aspect of 24-hour cocultures with endothelium were resuspended in control medium with or without added VIC7 (VIC7 was included in the apical compartment when it was also used in the basal compartment) and added to the upper chamber of chemotaxis wells. Stippled bars depict results obtained using uncoated 5.0-μm cellulose nitrate filters; solid bars show results using 8.0-μm collagen-coated filters. Data are representative of six experiments.