Effect of MoAbs against TF on reverse transendothelial migration of MP. PBMC were incubated with unstimulated HUVEC grown on collagen gels for 2 hours to allow accumulation of monocytes in the subendothelial collagen. Then cultures were washed to remove nonadherent cells, and fresh medium with or without MoAbs (20 μg/mL) was added. After 24 hours, the cultures were rinsed, and MoAb preparations were replenished. Cocultures of MP and HUVEC were incubated for a total of 48 hours, and then analyzed for reverse transmigration. Data are plotted relative to the percentage of reverse transmigration observed in the absence of added MoAb. Anti-TF MoAbs VIC7, HTF-K108, and HTF-K180 (all IgG1), and a variety of other IgG1 MoAbs, including TEA 1/31 against cadherin 5, F432G-3 against S-endo-4, and OJ79 against MUC18, were tested in a single screening experiment (A). The effect of anti-TF MoAb VIC7 was compared with that of anti-CD11/CD18 MoAb IB4 in three experiments (B). Another group of anti-TF MoAbs and an anti–factor VII MoAb were also evaluated in three experiments (C). Statistical differences relative to controls with no MoAb are denoted as (*), P < .02; (**), P < .005.