Fig. 9.
ICAM-1 expression is not modulated by Et-1. CNS-EC cultures were exposed to Et-1 (10−7 mol/L) or TNF (0.1 ng/mL) or left untreated for 18 hours. The cells were subsequently resuspended and incubated with anti–ICAM-1 and FITC-conjugated anti-mouse antibody consecutively. Labeled cells were analyzed in a FACS sorter, and the number of FITC-positive cells was determined. Data are the number of positive cells divided by the total number of cells counted (5,000 cells per sample).
