DNA sequence immediately 5′ of the β-globin gene in the Greek Cypriot family. The individuals II1, I1, I2, II2, and II3 (as shown in Fig 2) are represented by lanes 1, 2, 3, 4, and 5. The termination reactions are loaded in consecutive lanes in a block, ddGTP (G), ddATP (A), ddTTP (T), and ddCTP (C). (Panel A) The 5′ breakpoint indicated by the 1-bp deletion (arrowed) at position −911; (Panel B) the 3′ breakpoint indicated by the arrow (Rsa I site) at position −550.