A MUTZ-2 cell sample was stained with CD34(PE-Cy5), AC133(PE) as well as isotype control antibodies conjugated with PE-Cy5 or PE. (Top left) The gate on forward versus side scatter (1) contained the leukemic blast cell population (10% probability dot plot). (Top right) Expression of CD34 and AC133 on gated (R1) cells is shown. (Bottom left) The pattern of IgG-PE-Cy5 and IgG-PE isotype MoAb expression on the R1 population.