Fig. 3.
Fig. 3. Characterization of the recombinant proteins binding to ELISA plates and to heparin. (A) Amount of protein bound to individual wells as indicated by the sum total of relative binding to a PF4 polyclonal antibody (with PF4 given a value of 1) plus relative binding to a NAP-2 polyclonal antibody (with NAP-2 given a value of 1). (B) Amount of 3H-heparin bound to equal amounts of various recombinant proteins in an ELISA well. Protein 1 = WT PF4; 2 = NPPP; 3 = NNP*P; 4 = NNNP; 5 = PF4, K62; 6 = PF4, K66; 7 = WT NAP-2; and 8 = no recombinant protein.

Characterization of the recombinant proteins binding to ELISA plates and to heparin. (A) Amount of protein bound to individual wells as indicated by the sum total of relative binding to a PF4 polyclonal antibody (with PF4 given a value of 1) plus relative binding to a NAP-2 polyclonal antibody (with NAP-2 given a value of 1). (B) Amount of 3H-heparin bound to equal amounts of various recombinant proteins in an ELISA well. Protein 1 = WT PF4; 2 = NPPP; 3 = NNP*P; 4 = NNNP; 5 = PF4, K62; 6 = PF4, K66; 7 = WT NAP-2; and 8 = no recombinant protein.

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