Profiles of cytokines released by anti-CD3–stimulated T lymphocytes from mice receiving Salmonella-based vaccines. Spleen T lymphocytes were isolated and nylon-wool purified 45 days after primary vaccine administration and plated in the presence of immobilized anti-CD3 MoAb for 24 or 48 hours, respectively, to measure IFN-γ and IL-4 release. Cytokine levels were determined by sandwich ELISA.